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Given our relatively small samples and therefore limited power, however, further research could usefully examine if testosterone in saliva samples collected during the peak of mini-puberty in larger groups predicts autistic traits or other traits that show gender differences. Studying the causal mechanisms involved in this gender bias may provide insights into the aetiology of ASCs.

When the children (40 boys, 47 girls) reached 18 to 30 months of age, parents completed the Quantitative Checklist for Autism in Toddlers (Q-CHAT).

Boys had higher concentrations of testosterone postnatally and higher Q-CHAT scores than girls.

However, testosterone did not correlate with Q-CHAT scores in boys, girls, or the entire sample.